Difference between revisions of "Part:BBa K1316004"
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Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. | Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. | ||
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| + | <h3>Characterisation</h3> | ||
The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316005. Its response in front of 2,4-DNT, nevertheless, is much lower than the one of the yqjF promoter (BioBrick BBa_K1316003) as it can be seen on the characterisation of BioBrick BBa_K1316005 (https://parts.igem.org/Part:BBa_K1316005). | The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316005. Its response in front of 2,4-DNT, nevertheless, is much lower than the one of the yqjF promoter (BioBrick BBa_K1316003) as it can be seen on the characterisation of BioBrick BBa_K1316005 (https://parts.igem.org/Part:BBa_K1316005). | ||
Revision as of 13:37, 15 October 2014
ybiJ promoter
Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.
Characterisation
The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316005. Its response in front of 2,4-DNT, nevertheless, is much lower than the one of the yqjF promoter (BioBrick BBa_K1316003) as it can be seen on the characterisation of BioBrick BBa_K1316005 (https://parts.igem.org/Part:BBa_K1316005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 381
- 1000COMPATIBLE WITH RFC[1000]