Difference between revisions of "Part:BBa K1316001"

Revision as of 11:43, 15 October 2014

mKate2 protein with double transcription terminator

mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. BBa_K823017 Double Terminator (B0012-B0011)

This Composite part was used for the construction of the final BioBricks BBa_K1316003, BBa_K1316005, BBa_K1316007, BBa_K1316008 and BBa_K1316009.

The functionality of mKate2 is proven by the red fluorescence detected on the characterisation of BioBrick BBa_K1316003 (https://parts.igem.org/Part:BBa_K1316003).

The advantage of adding the double terminator has not been proven because the constructs containing the N-genes (BBa_K1316007 and BBa_K1316008) and the construct having a combination of both ybiJ and yqjF promoters (BBa_K1316009) did not seem to be properly induced by the presence of 2,4-DNT.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 454
Illegal BsaI.rc site found at 643

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1316001 short</partinfo>
-mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.
+mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in ''E. coli''.
 BBa_K823017 Double Terminator (B0012-B0011)