Difference between revisions of "Part:BBa K1493000"
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<partinfo>BBa_K1493000 short</partinfo> | <partinfo>BBa_K1493000 short</partinfo> | ||
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Promoter fusaric acid inducible | Promoter fusaric acid inducible | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | A fusaric acid efflux pump within ''Pseudomonas putida'' is encoded by an operon consisting of four genes. This operon is controlled by a LysR-type gene (pp1262) which is located upstream of the operon. This gene inhibits the binding of RNA polymerase to the promoter in the intergenic region between pp1262 and the operon. Fusaric acid will block this inhibition allowing activity of the operon. (See figure 1) Hence, pp1262 and the intergenic region will be isolated and put into BioBrick form, effectively acting as a Fusaric Acid inducible Promoter (FAiP). | ||
| + | https://static.igem.org/mediawiki/2014/f/f3/Wageningen_UR_sensing_Faip1.jpg<br> | ||
| + | ''Figure 1. Fusaric acid efflux pump operon present in the genome of KT2440 Pseudomonas putida.'' | ||
| − | + | ===Features=== | |
| − | + | This biobrick can only be used in ''Pseudomonas putida'', since the regulatory gene has been disrupted. | |
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| − | + | ===References=== | |
| − | === | + | Hu, R.-M., et al., An Inducible Fusaric Acid Tripartite Efflux Pump Contributes to the Fusaric Acid Resistance in Stenotrophomonas maltophilia. PLoS ONE, 2012. 7(12): p. e51053. |
| − | + | Nelson, K.E., et al., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental microbiology, 2002. 4(12): p. 799-808. | |
| − | + | </p> | |
Revision as of 11:32, 15 October 2014
Fusaric acid induced regulatory promoter
Promoter fusaric acid inducible
Usage and Biology
A fusaric acid efflux pump within Pseudomonas putida is encoded by an operon consisting of four genes. This operon is controlled by a LysR-type gene (pp1262) which is located upstream of the operon. This gene inhibits the binding of RNA polymerase to the promoter in the intergenic region between pp1262 and the operon. Fusaric acid will block this inhibition allowing activity of the operon. (See figure 1) Hence, pp1262 and the intergenic region will be isolated and put into BioBrick form, effectively acting as a Fusaric Acid inducible Promoter (FAiP).
Figure 1. Fusaric acid efflux pump operon present in the genome of KT2440 Pseudomonas putida.
Features
This biobrick can only be used in Pseudomonas putida, since the regulatory gene has been disrupted.
References
Hu, R.-M., et al., An Inducible Fusaric Acid Tripartite Efflux Pump Contributes to the Fusaric Acid Resistance in Stenotrophomonas maltophilia. PLoS ONE, 2012. 7(12): p. e51053. Nelson, K.E., et al., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental microbiology, 2002. 4(12): p. 799-808.