Difference between revisions of "Part:BBa K1462000:Design"
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | COXVI is obtained by PCR technique from Saccharomyces cerevisiae's genome. | + | COXVI is obtained by PCR technique from Saccharomyces cerevisiae's genome.<br/> |
| − | PCR system(KOD FX from Toyobo) | + | PCR system(KOD FX from Toyobo)<br/> |
| − | contents volume/μL | + | contents volume/μL<br/> |
------------------------------ | ------------------------------ | ||
| − | KOD FX 2×Buf 25 | + | KOD FX 2×Buf 25<br/> |
| − | dNTPS 10 | + | dNTPS 10<br/> |
| − | H₂O 8 | + | H₂O 8<br/> |
| − | Foward primer 2 | + | Foward primer 2<br/> |
| − | Reverse primer 2 | + | Reverse primer 2<br/> |
| − | templete 1 | + | templete 1<br/> |
| − | KOD FX 2 | + | KOD FX 2<br/> |
------------------------------- | ------------------------------- | ||
| − | total 50 | + | total 50<br/> |
Latest revision as of 09:45, 15 October 2014
CoxVI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
COXVI is obtained by PCR technique from Saccharomyces cerevisiae's genome.
PCR system(KOD FX from Toyobo)
contents volume/μL
KOD FX 2×Buf 25
dNTPS 10
H₂O 8
Foward primer 2
Reverse primer 2
templete 1
KOD FX 2
total 50
Source
CoxVI is obtained from Saccharomyces cerevisiae's genome by PCR techniques