Difference between revisions of "Part:BBa K1341001"

Latest revision as of 08:26, 15 October 2014

PATH WAY TEST SYS IN BIO-COMPASS

This is a measurement system comprised of a switch device(output GFP as signal)and a specialized path way sequence.This system can prove that we have insert the BBa_K1341000 in the testing path way successfully(we designed a AFL III Restriction Enzyme cutting site in the No.5 node sequence. Before we insert the device, we linked the matching AFL III Restriction Enzyme cutting site sequence on switch device's(BBa_K1341000)ends by using BioBrick assembly method.).Besides, this system also has a BamH I Restriction Enzyme cutting site in the No.2(start) node's sequence, a Nco I Restriction Enzyme cutting site in the No.8(terminal） node's sequence. With the same method, the TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can test whether the path way is the right result exactly such as this(2-4-5-7-8).

characterization

The analysis about the logic of the system

We can prove that the GFP signal part(BBa_K1341000) of the screen device has been insert in our path way test system((BBa_K1341000 is almost 1000bp, and the path way sequence is about 200 bp, this Electrophoresis result is about 1200bp), and we make this gene circuit(BBa_K1341001) do the follwing test about whether this path way circuit is the right way we want.

Sequence and Features