Difference between revisions of "Part:BBa K1416001"
Line 3: Line 3:
  
 
The part includes the gene coding for the <i>Methanocaldococcus jannaschii</i> synthetase that charges the orthogonal tRNA (also in the part) with the amino acid 3-iodotyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0".
 
The part includes the gene coding for the <i>Methanocaldococcus jannaschii</i> synthetase that charges the orthogonal tRNA (also in the part) with the amino acid 3-iodotyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0".
 +
 +
This part was used by the UT Austin 2014 iGEM team in their [http://2014.igem.org/Team:Austin_Texas/kit#Results_and_Data ncAA kit project]. In this work they demonstrated that this part was one of the best ncAA tRNA synthetase/tRNA pairs with an "incorporation value" of ~12.  This value indicates that this part has both high fidelity for 3-iodotyrosine incorporation as well as high efficiency.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:46, 14 October 2014

The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

The part includes the gene coding for the Methanocaldococcus jannaschii synthetase that charges the orthogonal tRNA (also in the part) with the amino acid 3-iodotyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0".

This part was used by the UT Austin 2014 iGEM team in their [http://2014.igem.org/Team:Austin_Texas/kit#Results_and_Data ncAA kit project]. In this work they demonstrated that this part was one of the best ncAA tRNA synthetase/tRNA pairs with an "incorporation value" of ~12. This value indicates that this part has both high fidelity for 3-iodotyrosine incorporation as well as high efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316
    Illegal SapI.rc site found at 1109