Difference between revisions of "Part:BBa K518010:Experience"

Revision as of 22:58, 14 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K518010

User Reviews

UNIQb19b420e1b6d2bfb-partinfo-00000000-QINU UNIQb19b420e1b6d2bfb-partinfo-00000001-QINU

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 <I>Cooper Union iGEM 2014</I>
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-We used this part to design a composite part, <partinfo>BBa_K1354000</partinfo>, with this promoter and a phage activator, <partinfo>BBa_I746352</partinfo>. This phage activator turned on a phage promoter with a GFP reporter composite part, <partinfo>BBa_I746321</partinfo>. The two composite parts were in separate plasmids.
+We used this part to design a composite part, <partinfo>BBa_K1354000</partinfo>, with this promoter and a phage activator, <partinfo>BBa_I746352</partinfo>. This phage activator turned on a phage promoter with a GFP reporter composite part, <partinfo>BBa_I746321</partinfo>. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below.
 [[File:CU_UV_promoter_graph.JPG]]
 [[File:CU_UV_promoter_table.JPG]]