Difference between revisions of "Part:BBa K1462520:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"<bbpart>BBa_K1462490<bbpart/>,"GAL1 + GFP + BFP ligand + ADH1"<bbpart>BBa_K1462500<bbpart/> and <bbpart>BBa_K1462510<bbpart/>.  We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein.  
+
This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"[<bbpart>BBa_K1462490<bbpart/>],"GAL1 + GFP + BFP ligand + ADH1"[<bbpart>BBa_K1462500<bbpart/>] and GAL1 + YFP + SH3 ligand + ADH1[<bbpart>BBa_K1462510<bbpart/>].  We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein.  
  
  
 
===Source===
 
===Source===
−
This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"<bbpart>BBa_K1462490<bbpart/>,"GAL1 + GFP + BFP ligand + ADH1"<bbpart>BBa_K1462500<bbpart/> and <bbpart>BBa_K1462510<bbpart/>.
+
This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"[<bbpart>BBa_K1462490<bbpart/>],"GAL1 + GFP + BFP ligand + ADH1"[<bbpart>BBa_K1462500<bbpart/>] and GAL1 + YFP + SH3 ligand + ADH1[<bbpart>BBa_K1462510<bbpart/>].
  
 
===References===
 
===References===

Revision as of 18:16, 14 October 2014

GAL1+YFP+SH3-lig+ADH1+GAL1+GFP+PDZ-lig+ADH1+GAL1+BFP+GBD-lig+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1755
    Illegal AgeI site found at 3300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2804


Design Notes

This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"[BBa_K1462490