Difference between revisions of "Part:BBa K1462520:Design"

Revision as of 18:00, 14 October 2014

GAL1+YFP+SH3-lig+ADH1+GAL1+GFP+PDZ-lig+ADH1+GAL1+BFP+GBD-lig+ADH1

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
Illegal AgeI site found at 1755
Illegal AgeI site found at 3300
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2804

Design Notes

This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"BBa_K1462490

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 __NOTOC__
 <partinfo>BBa_K1462520 short</partinfo>
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 ===Design Notes===
-eee
+This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"<bbpart>BBa_K1462490<bbpart/>,"GAL1 + GFP + BFP ligand + ADH1"<bbpart>BBa_K1462500<bbpart/> and <bbpart>BBa_K1462510<bbpart/>.  We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein.
 ===Source===
+This device is composed of three parts "GAL1 + BFP + GBD ligand + ADH1"<bbpart>BBa_K1462490<bbpart/>,"GAL1 + GFP + BFP ligand + ADH1"<bbpart>BBa_K1462500<bbpart/> and <bbpart>BBa_K1462510<bbpart/>.
-fff
+===References===
-===References===
+[1] John E Dueber, Gabriel C Wu, G Reza Malmirchegini, et al.Synthetic protein scaffolds provide modular control
+over metabolic flux.Nat Biotechnol. 2009 Aug;27(8):753-9. <br/>
+[2] Schultz, J. et al. Specific interactions between the syntrophin PDZ domain and
+voltage-gated sodium channels. Nat Struct Biol 5, 19-24 (1998).<br/>
+[3] Kim, A.S., Kakalis, L.T., Abdul-Manan, N., Liu, G.A. & Rosen, M.K.
+Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome
+protein. Nature 404, 151-158 (2000).<br/>
+[4] Wu, X. et al. Structural basis for the specific interaction of lysine-containing
+proline-rich peptides with the N-terminal SH3 domain of c-Crk. Structure 3, 215-226 (1995).<br/>
+[5] Harris, B.Z., Hillier, B.J. & Lim, W.A. Energetic determinants of internal motif
+recognition by PDZ domains. Biochemistry 40, 5921-5930 (2001).<br/>
+[6] Dueber, J.E., Yeh, B.J., Chak, K. & Lim, W.A. Reprogramming control of an
+allosteric signaling switch through modular recombination. Science 301, 1904-1908 (2003).<br/>
+[7] Nguyen, J.T., Turck, C.W., Cohen, F.E., Zuckermann, R.N. & Lim, W.A.
+Exploiting the basis of proline recognition by SH3 and WW domains: design of
+N-substituted inhibitors. Science 282, 2088-2092 (1998).<br/>