Difference between revisions of "Part:BBa K1529300"
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<partinfo>BBa_K1529300 short</partinfo> | <partinfo>BBa_K1529300 short</partinfo> | ||
− | We newly developed the Prhl(RL) promoter (<partinfo>BBa_K1529300</partinfo>) which is activated by C4HSL (Fig. 1). <br> | + | We newly developed the Prhl(RL) promoter (<partinfo>BBa_K1529300</partinfo>) by improving Prhl promoter (<partinfo>BBa_R0071</partinfo>) which is activated by C4HSL (Fig. 1). <br> |
To characterize the function of this Prhl(RL) promoter, we constructed a part, Prhl(RL)-GFP (<partinfo>BBa_K1529301</partinfo>), by inserting the Prhl(RL) promoter, upstream of a GFP coding sequence. | To characterize the function of this Prhl(RL) promoter, we constructed a part, Prhl(RL)-GFP (<partinfo>BBa_K1529301</partinfo>), by inserting the Prhl(RL) promoter, upstream of a GFP coding sequence. | ||
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[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]] | [[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]] | ||
− | By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. | + | By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.) <br> |
− | We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay | + | We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay |
[[Image:Improved_Prhl_Promoter_Assay_Result.png|thumb|center|600px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]] | [[Image:Improved_Prhl_Promoter_Assay_Result.png|thumb|center|600px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]] |
Revision as of 02:17, 14 October 2014
Prhl(RL)
We newly developed the Prhl(RL) promoter (BBa_K1529300) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL (Fig. 1).
To characterize the function of this Prhl(RL) promoter, we constructed a part, Prhl(RL)-GFP (BBa_K1529301), by inserting the Prhl(RL) promoter, upstream of a GFP coding sequence.
By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay
For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]