Difference between revisions of "Part:BBa K1439001"

(→‎Result)
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Conjugate between E.coil HB101 and E.coil Top10.
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Conjugate between ''E.coil'' HB101 and ''E.coil'' Top10.

Revision as of 02:09, 14 October 2014

This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 30
    Illegal AgeI site found at 1139
    Illegal AgeI site found at 1251
  • 1000
    COMPATIBLE WITH RFC[1000]



Result

We do conjugation experiment between HB101 and Top10 to test the conjugation ability of OriTRP4 (BBa_K1439000). The HB101 strain, sensitive to streptomycin, is provided by QIU Zhigang. And Top10 has streptomycin resistance. In order to test BBa_K1439000 better, we ligate OriTRP4 with the report gene RFP. After conjugation, we pick the red colonies by LB medium with resistance.


Conjugate between E.coil HB101 and E.coil Top10.