Difference between revisions of "Part:BBa K1439001:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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OriTRP4 region contains some promoters, we put it in the back of reporter gene . In order to reduce the impact on protein expression
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For checking conjugation
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−
 
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===Source===
 
===Source===

Latest revision as of 02:01, 14 October 2014


This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 30
    Illegal AgeI site found at 1139
    Illegal AgeI site found at 1251
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For checking conjugation

Source

OriTRP4 comes form the RP4 plasmid, from 51098bp to 51448bp, reporter gene comes from BBa_J04450.

References