Difference between revisions of "Part:BBa K1401007"
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The promoter can be induced by anhydrotetracycline (aTc) and arabinose, as shown below. In order to test this tandem promoter, we built a basic transcriptional unit with red fluorescent protein reporter. We used the ''E. coli'' DH5-alpha Pro strain for this study, which has ''tetR'', ''lacI'', and ''araC'' all constitutively expressed in its genome. We induced the E. coli with aTc alone, arabinose alone, and then both aTc and arabinose together to see if we could determine if one promoter was dominant over the other. | The promoter can be induced by anhydrotetracycline (aTc) and arabinose, as shown below. In order to test this tandem promoter, we built a basic transcriptional unit with red fluorescent protein reporter. We used the ''E. coli'' DH5-alpha Pro strain for this study, which has ''tetR'', ''lacI'', and ''araC'' all constitutively expressed in its genome. We induced the E. coli with aTc alone, arabinose alone, and then both aTc and arabinose together to see if we could determine if one promoter was dominant over the other. | ||
| − | [[File:K1401007_flow.png|center| | + | [[File:K1401007_flow.png|center|600px]] |
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Revision as of 00:17, 14 October 2014
Tandem promoter pTet-pBad
This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This tandem promoter is in pSB1C3.
The promoter can be induced by anhydrotetracycline (aTc) and arabinose, as shown below. In order to test this tandem promoter, we built a basic transcriptional unit with red fluorescent protein reporter. We used the E. coli DH5-alpha Pro strain for this study, which has tetR, lacI, and araC all constitutively expressed in its genome. We induced the E. coli with aTc alone, arabinose alone, and then both aTc and arabinose together to see if we could determine if one promoter was dominant over the other.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 324
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 159
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 141