Difference between revisions of "Part:BBa K1401007"
Line 6: Line 6:
 
This tandem promoter has chloramphenicol resistance as it is located in pSB1C3. The promoter is induced by anhydrotetracycline and arabinose.
 
This tandem promoter has chloramphenicol resistance as it is located in pSB1C3. The promoter is induced by anhydrotetracycline and arabinose.
  
−
[[File:K1401007_flow.png]]
+
<center><img src="https://static.igem.org/mediawiki/parts/c/cd/K1401007_flow.png" width="600px"></center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:04, 14 October 2014

Tandem promoter pTet-pBad

This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

This tandem promoter has chloramphenicol resistance as it is located in pSB1C3. The promoter is induced by anhydrotetracycline and arabinose.

<img src="K1401007_flow.png" width="600px">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 159
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 141