Difference between revisions of "Part:BBa K1401007"

Revision as of 00:01, 14 October 2014

Tandem promoter pTet-pBad

This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

This tandem promoter has chloramphenicol resistance as it is located in pSB1C3. The promoter is induced by anhydrotetracycline and arabinose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 324
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 159
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 141

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1401007 short</partinfo>
-This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This transcriptional unit has Kanamycin resistance. The promoter is induced by atc and arabinose.
+This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
+This tandem promoter has chloramphenicol resistance as it is located in pSB1C3. The promoter is induced by anhydrotetracycline and arabinose.
-The characterization data will be updated before the jamboree.
+[[File:K1401007_flow.png]]
 <!-- Add more about the biology of this part here