Difference between revisions of "Part:BBa K1529300"
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We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).
 
We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).
  
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[[Image:titech2014_parts_GFP_assay_main_Fig.3.jpg|thumb|center|550px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]]
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[[Image:Improved_Prhl_Promoter_Assay_Result.png|thumb|center|600px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]]
  
 
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Revision as of 16:46, 13 October 2014

Prhl(RL)

We newly developed the Prhl(RL) promoter (BBa_K1529300) which is activated by C4HSL (Fig. 1).
To characterize the function of this Prhl(RL) promoter, we constructed a part, Prhl(RL)-GFP (BBa_K1529301), by inserting the Prhl(RL) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter
Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).
We saw that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).

Fig. 3. Fluorescence intensity detected by flow cytometer


For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]