Difference between revisions of "Part:BBa K1321011:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1321011 short</partinfo>
 
<partinfo>BBa_K1321011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Codon optimised for E.coli. rbs not included in the part as it is for fusions
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The DNA sequence from [https://parts.igem.org/Part:BBa_K519010 BBa_K519010] was codon optimised using the propriety software from GeneArt®. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
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===Source===
 
===Source===
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The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).
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Source from BBa_____
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===References===
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Latest revision as of 15:49, 13 October 2014

SmtA metallothionein in Freiburg format (RFC[25])


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence from BBa_K519010 was codon optimised using the propriety software from GeneArt®. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.

Source

The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).