Difference between revisions of "Part:BBa J54103:Design"

 
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===Design Notes===
 
===Design Notes===
−
as negative control
+
We use this as negative control.
−
 
+
This prasmid is the basic part to insert one or several protein_binding_sites.
−
 
+
  
 
===Source===
 
===Source===
  
−
synthesis
+
iGEM part BBa_I7100.
 +
We deleted the promoter and inserted newly designed prefix and suffix each of which has 2 restriction-enzyme-sites(prefix: SalI and BamHI,surfix: BglII and MulI)
 +
But we did not insert new promoter.
  
 
===References===
 
===References===

Latest revision as of 04:26, 22 October 2006


Promoterless-GFP(A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 27
    Illegal BamHI site found at 15
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 714


Design Notes

We use this as negative control. This prasmid is the basic part to insert one or several protein_binding_sites.

Source

iGEM part BBa_I7100. We deleted the promoter and inserted newly designed prefix and suffix each of which has 2 restriction-enzyme-sites(prefix: SalI and BamHI,surfix: BglII and MulI) But we did not insert new promoter.

References