Difference between revisions of "Part:BBa K1321339:Design"
 
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Nucleotide sequence was first obtained from the relevant portion of [http://www.ebi.ac.uk/ena/data/view/M15823 ENI M15823.1], codon optimised for ''E. coli'' and modified to reduce GC content. The DNA sequence for RFC25 prefix and suffix was appeneded to the sequence, with an additional 4 basepairs (gatc) at the beginning and end of the sequence to allow for space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
 
Nucleotide sequence was first obtained from the relevant portion of [http://www.ebi.ac.uk/ena/data/view/M15823 ENI M15823.1], codon optimised for ''E. coli'' and modified to reduce GC content. The DNA sequence for RFC25 prefix and suffix was appeneded to the sequence, with an additional 4 basepairs (gatc) at the beginning and end of the sequence to allow for space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
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===Source===
 
===Source===
  
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The designed construct was ordered from as a GeneArt (R) String (Invitrogen TM Life Technologies)
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The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).

Latest revision as of 14:47, 13 October 2014

CBDcenA+Linker, RFC 25 standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide sequence was first obtained from the relevant portion of [http://www.ebi.ac.uk/ena/data/view/M15823 ENI M15823.1], codon optimised for E. coli and modified to reduce GC content. The DNA sequence for RFC25 prefix and suffix was appeneded to the sequence, with an additional 4 basepairs (gatc) at the beginning and end of the sequence to allow for space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.

Source

The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).