Difference between revisions of "Part:BBa K1355000"
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<partinfo>BBa_K1355000 short</partinfo>
 
<partinfo>BBa_K1355000 short</partinfo>
  
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The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system.
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The MerA gene is one of the most important gene into bacterial mercury-resistance operon. The mercuric reductase is a cytosolic flavin disulfide oxidoreductase (homodimer ∼120 kDa) which uses NAD(P)H as a reductant. Transforms mercury Hg2+ in Hg0, which volatile, lipid-soluble Hg(0) diffuses through the cell membranes without the need for any dedicated efflux system, as shown in figure below.  
  
  
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Figure 01: Mer genes action in bacterial cell
 
Figure 01: Mer genes action in bacterial cell
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[[File:l2.png]]
 
[[File:l2.png]]
  
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Figure 02: MerA protein 3D Structure
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Figure 02: MerA homodimer protein 3D structure
  
 
== Source  ==
 
== Source  ==

Revision as of 14:44, 13 October 2014

Strong RBS + merA (mercuric ion reductase)+ terminator (BBa_ B0015)

The MerA gene is one of the most important gene into bacterial mercury-resistance operon. The mercuric reductase is a cytosolic flavin disulfide oxidoreductase (homodimer ∼120 kDa) which uses NAD(P)H as a reductant. Transforms mercury Hg2+ in Hg0, which volatile, lipid-soluble Hg(0) diffuses through the cell membranes without the need for any dedicated efflux system, as shown in figure below.


L1.png

Figure 01: Mer genes action in bacterial cell


L2.png

Figure 02: MerA homodimer protein 3D structure

Source

MerA gene sequence is found in the O26-CRL plasmid from Escherichia coli O26. We also added strong RBS from protein 10 found in phage 17 and the double terminator (BBa_B0015) to ensure efficient termination of transcription and messenger RNA recognition.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1200
    Illegal NgoMIV site found at 1262
  • 1000
    COMPATIBLE WITH RFC[1000]