Difference between revisions of "Part:BBa K1537015"
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| − | == | + | == 35S promoter == |
| − | The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively. | + | The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively.( Kuluev et al, 2010) |
| − | + | ==Translation initiation optimized sequence for dicot == | |
| − | == | + | |
We use the nucleotide sequence to enhance translation initiation(Samir V. Sawant et al 2001). | We use the nucleotide sequence to enhance translation initiation(Samir V. Sawant et al 2001). | ||
| − | == | + | == Mass translation enhancer == |
Insertion of “GCT TCC TCC” after the initiator codon ATG can augment downstream gene-expression in plants(So Nakagawa et al 2008). | Insertion of “GCT TCC TCC” after the initiator codon ATG can augment downstream gene-expression in plants(So Nakagawa et al 2008). | ||
| + | |||
References | References | ||
Latest revision as of 12:19, 13 October 2014
35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer
35S promoter
The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively.( Kuluev et al, 2010)
Translation initiation optimized sequence for dicot
We use the nucleotide sequence to enhance translation initiation(Samir V. Sawant et al 2001).
Mass translation enhancer
Insertion of “GCT TCC TCC” after the initiator codon ATG can augment downstream gene-expression in plants(So Nakagawa et al 2008).
References
Kuluev, B. R., Knyazev, A. V., Lebedev, P. Ya., Iljassowa, A. A. and Chemeris, A. V.(2010) Construction of Hybrid Promoters of Caulimoviruses and Analysis of Their Activity in Transgenic Plants. Russian Journal of Plant Physiology ,Vol. 57, No. 4, 582-589. So Nakagawa, Yoshihito Niimura, Takashi Gojobori,Hiroshi Tanaka and Kin-ichiro Miura(2008) Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Nucl. Acids Res. 36 (3): 861-871. Samir V. Sawant, Kanti Kiran, Pradhyumna K. Singh, and Rakesh Tuli(2001)Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants. Plant Physiology 126(4) 1630-1636.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 66