Difference between revisions of "Part:BBa K1529300"
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We newly developed the <i>rhl(RL)</i> promoter (<partinfo>BBa_K1529300</partinfo>) which is activated by C4HSL (Fig. 1). <br>
 
We newly developed the <i>rhl(RL)</i> promoter (<partinfo>BBa_K1529300</partinfo>) which is activated by C4HSL (Fig. 1). <br>
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To characterize the function of this <i>rhl(RL)</i> promoter, we constructed a part Prhl(RL)_GFP (<partinfo>BBa_K1529301</partinfo>) by inserting the <i>rhl(RL)</i> promoter, upstream of a GFP coding sequence.
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To characterize the function of this <i>rhl(RL)</i> promoter, we constructed a part Prhl(RL)-GFP (<partinfo>BBa_K1529301</partinfo>) by inserting the <i>rhl(RL)</i> promoter, upstream of a GFP coding sequence.
  
 
[[Image:titech2014_parts_rhl_promoter_main_Fig1.png|thumb|center|550px|<b>Fig. 1.</b> Our newly designed  promoter]]
 
[[Image:titech2014_parts_rhl_promoter_main_Fig1.png|thumb|center|550px|<b>Fig. 1.</b> Our newly designed  promoter]]
 
[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]]
 
[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]]
  
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By using the reporter cells that contain Prhl(RL)_GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).  
+
By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).  
 
We saw that our new <i>rhl(RL)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 2).
 
We saw that our new <i>rhl(RL)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 2).
  

Revision as of 07:36, 13 October 2014

Prhl(RL)

We newly developed the rhl(RL) promoter (BBa_K1529300) which is activated by C4HSL (Fig. 1).
To characterize the function of this rhl(RL) promoter, we constructed a part Prhl(RL)-GFP (BBa_K1529301) by inserting the rhl(RL) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter
Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2). We saw that our new rhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).

Fig. 3. Fluorescence intensity detected by flow cytometer


For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]