Difference between revisions of "Part:BBa K1381008"
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The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032). | The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032). | ||
| − | Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part: | + | Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K1381000 BBa_K1381000] (yenbox_WT). |
Revision as of 20:37, 12 October 2014
yenbox_WT-B0032-GFP
This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K138004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.
The BioBrick that were used for assembling this construct was BBa_K138000 (yenbox_WT) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032).
Too read more about the Yen system and for the results from the characterisation experiments, see BBa_K1381004 (B0034-YenR) or BBa_K1381000 (yenbox_WT).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 41
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 753