Difference between revisions of "Part:BBa K1396001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1396001 short</partinfo> | <partinfo>BBa_K1396001 short</partinfo> | ||
− | + | The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. | |
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+ | This is an improvement on the Utah State biobrick [https://parts.igem.org/Part:BBa_K1162006 BBa_K1162006] which consists of only the LL-37 peptide. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:49, 12 October 2014
pSB1C3: LL-37_MFP
The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action.
This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 232
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]