Difference between revisions of "Part:BBa K1381019"

Revision as of 15:58, 12 October 2014

B0034-pelB-CFY-X6 his

Colicin Fy The CFY is a bacteriocin in the colicin family. It is produced naturally by Y.frederiksenii and target close relatives to that bacteria. One among them is the Y.enterocolitica and our target pathogene. The Colicin Fy will target the cellmembrane of its target.

PelB PelB is en export tag that works in E.coli.

The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, PelB. The part contains the colicin FyBBa_K1381017, the export tag for E.coli PelB and a RBS. This part do not have any promoter. It is desgined that way so it can be easier to couple a promoter of your choice with the rest of the killing system. However due to problems with the restrictions enzyme for AgeI and MgoMIV the Uppsala iGEM team 2014 could not coupled the export tag, pelB and the colicin Fy together with the Freiburg Biobrick standard 25. Instead we used the regular Biobrick standard 10 to couple them which results in a stop codon between pelB and CFY. For this part to work, the stop codon must be removed with mutagenes. This part also contains an 6x His-Tag for characterisation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 76
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 '''PelB''' PelB is en export tag that works in ''E.coli''.
-'''The killing system''' We designed the killing system to secret the colicin Fy with the help of an export tag, PelB. The part contains the colicin Fy[https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017], the export tag for ''E.coli'' PelB and a RBS. This part do not have any promoter. It is desgined that way so it can be easier to couple a promoter of your choice with the rest of the killing system. However due to problems with the restrictions enzyme for AgeI and MgoMIV the Uppsala iGEM team 2014 could not coupled the export tag, pelB and the colicin Fy together with the Freiburg Biobrick standard 25. Instead we used the regular Biobrick standard 10 to couple them which results in a stop codon between pelB and CFY. For this part to work, the stop codon must be removed with mutagenes.
+'''The killing system''' We designed the killing system to secret the colicin Fy with the help of an export tag, PelB. The part contains the colicin Fy[https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017], the export tag for ''E.coli'' PelB and a RBS. This part do not have any promoter. It is desgined that way so it can be easier to couple a promoter of your choice with the rest of the killing system. However due to problems with the restrictions enzyme for AgeI and MgoMIV the Uppsala iGEM team 2014 could not coupled the export tag, pelB and the colicin Fy together with the Freiburg Biobrick standard 25. Instead we used the regular Biobrick standard 10 to couple them which results in a stop codon between pelB and CFY. For this part to work, the stop codon must be removed with mutagenes. This part also contains an 6x His-Tag for characterisation.
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