Difference between revisions of "Part:BBa K1442111"

 
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<partinfo>BBa_K1442111 short</partinfo>
 
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As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.
 
  
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== DPP-IV ==
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===Usage and Biology===
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As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).
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[[File:DPPIV.jpg]]
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<span class='h3bb'>Sequence and Features</span>
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''The schematic above shows the organisation of the DPP-IV target''
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<partinfo>BBa_K1442111 SequenceAndFeatures</partinfo>
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== Usage ==
  
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This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.
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===Functional Parameters===
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<partinfo>BBa_K1442111 parameters</partinfo>
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Revision as of 15:48, 12 October 2014

DPP-IV (our siRNA target)


DPP-IV

As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).


DPPIV.jpg The schematic above shows the organisation of the DPP-IV target

Usage

This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.