Difference between revisions of "Part:BBa K1403019:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1403019 short</partinfo>
 
<partinfo>BBa_K1403019 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
This sequence was submitted downstream a standard T7 constitutive promoter inside a pETDuet vector.
 
This sequence was submitted downstream a standard T7 constitutive promoter inside a pETDuet vector.
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The ilvBN sequence was isolated from MG1655 through PCR and digested with NCoI and EcoRI to put into pETDUET1 vector. It was transformed into E.Coli strain with plasmid containing aldB to produce acetoin, which has the smell of butter.
  
  
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===References===
 
===References===
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David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.

Revision as of 14:55, 12 October 2014

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was submitted downstream a standard T7 constitutive promoter inside a pETDuet vector.

The ilvBN sequence was isolated from MG1655 through PCR and digested with NCoI and EcoRI to put into pETDUET1 vector. It was transformed into E.Coli strain with plasmid containing aldB to produce acetoin, which has the smell of butter.


Source

E. coli MG1655

References

David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.