Difference between revisions of "Part:BBa K1403003:Design"
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The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library. | The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library. | ||
| − | We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. | + | We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. |
| − | + | ||
| + | The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format. | ||
===Source=== | ===Source=== | ||
Revision as of 13:46, 12 October 2014
Limonene synthase (LIMS1) expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Design Notes
The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.
We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later.
The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.
Source
Registry. BBa_I742110
References
Edinburg iGEM Project 2007