Difference between revisions of "Part:BBa K1403003:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1403003 short</partinfo>
 
<partinfo>BBa_K1403003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Standard iGEM cloning.
+
The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.
 +
 
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We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later.  
  
  
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===Source===
 
===Source===
  
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Registry.
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Registry. BBa_I742110
  
 
===References===
 
===References===
 +
 +
Edinburg iGEM Project 2007

Revision as of 13:44, 12 October 2014

Limonene synthase (LIMS1) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.

We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later.


Source

Registry. BBa_I742110

References

Edinburg iGEM Project 2007