Difference between revisions of "Part:BBa K1403003"
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<partinfo>BBa_K1403003 short</partinfo> | <partinfo>BBa_K1403003 short</partinfo> | ||
− | This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. | + | This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli. |
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Revision as of 13:38, 12 October 2014
Limonene synthase (LIMS1) expression cassette
This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Verification
Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator