Difference between revisions of "Part:BBa K1442102"

 
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<partinfo>BBa_K1442102 short</partinfo>
 
<partinfo>BBa_K1442102 short</partinfo>
  
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GFP oriented in the negative sense for use in E.coli. Combined with other parts to act as testing modules.
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== RGE ==
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Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.
  
 
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===Usage and Biology===
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===Usage ===
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== Usage ==
  
 
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Revision as of 13:31, 12 October 2014

Reverse GFP for E.coli

RGE

Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 763