Difference between revisions of "Part:BBa K1486025"
| Line 16: | Line 16: | ||
<FONT SIZE="2"><P> | <FONT SIZE="2"><P> | ||
| − | The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. | + | The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA. |
</P></FONT> | </P></FONT> | ||
'''Secondary Structure''' | '''Secondary Structure''' | ||
Revision as of 09:29, 12 October 2014
|
|
ADH1 Terminator The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae. |
Purpose and origin of the Biobrick
The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA.
Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]