Difference between revisions of "Part:BBa K1537026"
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | + | __NOTOC__ | |
| + | <partinfo>BBa_K1537026 short</partinfo> | ||
| + | |||
The glutathione-dependent formaldehyde dehydrogenase(FALDH) plays a key role in formaldehyde metabolism. FALDH is identified as an enzyme expressed in the cytoplasm. If we make FALDH over-express in plants, we can enhance plants’ tolerance to HCHO and increase the ability of plants to absorb HCHO. In the process of metabolism of formaldehyde, the formaldehyde may first combined with glutathione (GSH) to form the product of S-hydroxymethyl glutathione (HM-GSH), then FALDH in cytoplasm will catalyzes the formation of a S-formyl glutathione(F-GSH). Next the F-GSH will be hydrolyzed to formate(HCOOH) and GSH by S-formyl glutathione hydrolase (FGH). | The glutathione-dependent formaldehyde dehydrogenase(FALDH) plays a key role in formaldehyde metabolism. FALDH is identified as an enzyme expressed in the cytoplasm. If we make FALDH over-express in plants, we can enhance plants’ tolerance to HCHO and increase the ability of plants to absorb HCHO. In the process of metabolism of formaldehyde, the formaldehyde may first combined with glutathione (GSH) to form the product of S-hydroxymethyl glutathione (HM-GSH), then FALDH in cytoplasm will catalyzes the formation of a S-formyl glutathione(F-GSH). Next the F-GSH will be hydrolyzed to formate(HCOOH) and GSH by S-formyl glutathione hydrolase (FGH). | ||
| + | In paper” Overexpression of the Formaldehyde Dehydrogenase Gene from Brevibacillus brevis to Enhance Formaldehyde Tolerance and Detoxification of Tobacco”, the gaseous H13CHO metabolic spectrum in the transgenic and WT tobacco was analyzed using 13C-NMR. As shown in Fig.1 | ||
| + | |||
| + | [[File:faldh.jpg]] | ||
| + | |||
| + | |||
| + | Fig.1 a 13^C-NMR spectra from leaf extracts of transgenic tobacco plant treated with gaseous H^13 CHO for 2 h. b 13^C-NMR spectra from leaf extracts of WT treated with gaseous H^13 CHO for 2 h. c The extract from WT plant leaves without H^13 CHO treatment was used to monitor the background ^3 C-NMR signal levels. | ||
| + | |||
| + | References | ||
| + | |||
| + | Hongjuan Nian & Qingchao Meng &Wei Zhang &Limei Chen.(2013)Overexpression of the Formaldehyde Dehydrogenase Gene from Brevibacillus brevis to Enhance Formaldehyde Tolerance and Detoxification of Tobacco. Appl Biochem Biotechnol .169:170–180 | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1537026 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1537026 SequenceAndFeatures</partinfo> | ||
Latest revision as of 05:37, 12 October 2014
FALDH
The glutathione-dependent formaldehyde dehydrogenase(FALDH) plays a key role in formaldehyde metabolism. FALDH is identified as an enzyme expressed in the cytoplasm. If we make FALDH over-express in plants, we can enhance plants’ tolerance to HCHO and increase the ability of plants to absorb HCHO. In the process of metabolism of formaldehyde, the formaldehyde may first combined with glutathione (GSH) to form the product of S-hydroxymethyl glutathione (HM-GSH), then FALDH in cytoplasm will catalyzes the formation of a S-formyl glutathione(F-GSH). Next the F-GSH will be hydrolyzed to formate(HCOOH) and GSH by S-formyl glutathione hydrolase (FGH).
In paper” Overexpression of the Formaldehyde Dehydrogenase Gene from Brevibacillus brevis to Enhance Formaldehyde Tolerance and Detoxification of Tobacco”, the gaseous H13CHO metabolic spectrum in the transgenic and WT tobacco was analyzed using 13C-NMR. As shown in Fig.1
Fig.1 a 13^C-NMR spectra from leaf extracts of transgenic tobacco plant treated with gaseous H^13 CHO for 2 h. b 13^C-NMR spectra from leaf extracts of WT treated with gaseous H^13 CHO for 2 h. c The extract from WT plant leaves without H^13 CHO treatment was used to monitor the background ^3 C-NMR signal levels.
References
Hongjuan Nian & Qingchao Meng &Wei Zhang &Limei Chen.(2013)Overexpression of the Formaldehyde Dehydrogenase Gene from Brevibacillus brevis to Enhance Formaldehyde Tolerance and Detoxification of Tobacco. Appl Biochem Biotechnol .169:170–180 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]