Difference between revisions of "Part:BBa K1471006"

 
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<partinfo>BBa_K1471006 short</partinfo>
 
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RBS for yeast and a light receptor named phytochrome B.
 
RBS for yeast and a light receptor named phytochrome B.
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Phytochrome B is a photoreceptor protein which belongs to a multigeneti family that goes from Phy A to Phy E, every one of this genes is involved in a different function in the plant. This light-receptor protein is stimulated mostly for red-light and far red-light  in the espectrum at 700-800nm. Phytochrom B has the ability to absorb the redlight in order to be able to absorb the far red-light, this happens due to a conformation change in the protein which goes back to its initial state once it absorbs the far red-light. The previous conformations are known as Pr which is able to absorb the red-light and pfr that absorbs the far red-light.
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Phytochrome B is known to be involved in the seeds germination under certain light conditions, in fact mutants with absence of Phy B does not germinate in the same proportion as the wild type, 80% of the seeds tried to be germinated in absence of light did not show any results.
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https://static.igem.org/mediawiki/2014/1/13/Phytochrome_B.png  Figure no. 1
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In the figure no. 1 its shown the action of the protein under different red light emissions, Where the normal conformation of the protein is change by the red-light emission at 660 nm and then is able to absorb the far red-light and also interact with a specific molecule called phytochrome interacting fator 6 or "pif6" which acts as a signal transmisor from the Phytochrome B to other systems.
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Revision as of 01:11, 12 October 2014

RBS with PhyB.

RBS for yeast and a light receptor named phytochrome B.

Phytochrome B is a photoreceptor protein which belongs to a multigeneti family that goes from Phy A to Phy E, every one of this genes is involved in a different function in the plant. This light-receptor protein is stimulated mostly for red-light and far red-light in the espectrum at 700-800nm. Phytochrom B has the ability to absorb the redlight in order to be able to absorb the far red-light, this happens due to a conformation change in the protein which goes back to its initial state once it absorbs the far red-light. The previous conformations are known as Pr which is able to absorb the red-light and pfr that absorbs the far red-light.

Phytochrome B is known to be involved in the seeds germination under certain light conditions, in fact mutants with absence of Phy B does not germinate in the same proportion as the wild type, 80% of the seeds tried to be germinated in absence of light did not show any results.

Phytochrome_B.png Figure no. 1

In the figure no. 1 its shown the action of the protein under different red light emissions, Where the normal conformation of the protein is change by the red-light emission at 660 nm and then is able to absorb the far red-light and also interact with a specific molecule called phytochrome interacting fator 6 or "pif6" which acts as a signal transmisor from the Phytochrome B to other systems.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
    Illegal BamHI site found at 589
    Illegal XhoI site found at 540
    Illegal XhoI site found at 559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 756