Difference between revisions of "Part:BBa K1442006:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1442006 short</partinfo> | <partinfo>BBa_K1442006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | <p>This was BioBrick compatible therefore no alterations were required.</p> | |
+ | <p>MauBI and MluI sites were added in order to insert the aptazyme into the forwards GFP and EMCV. MauBI and MluI sites are compatible hence CIP must be added in cloning. </p> | ||
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===Source=== | ===Source=== | ||
− | http://nar.oxfordjournals.org/content/early/2013/04/12/nar.gkt253.full | + | <p><b>[1]</b><html><body><a href="http://nar.oxfordjournals.org/content/early/2013/04/12/nar.gkt253.full">Small engineered RNAs</a></body></html></p> |
+ | <p><b>[2]</b><html><body><a href="http://www.readcube.com/articles/10.1093/nar/gkt253">Klauser and Hartig (2012)</a></body></html></p> | ||
===References=== | ===References=== |
Latest revision as of 21:58, 11 October 2014
Anti-theophylline Aptazyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This was BioBrick compatible therefore no alterations were required.
MauBI and MluI sites were added in order to insert the aptazyme into the forwards GFP and EMCV. MauBI and MluI sites are compatible hence CIP must be added in cloning.
Source
[1]
Small engineered RNAs[2]
Klauser and Hartig (2012)