Difference between revisions of "Part:BBa K1442006"
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− | An aptazyme is | + | <p>An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.</p> |
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+ | One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 19:48, 11 October 2014
Anti-theophylline Aptazyme
An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.
One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]