Difference between revisions of "Part:pSB1A7:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | '''Purpose'''<br> | |
| − | + | Read-through transcription is extremely problematic when the usefulness of a device depends upon an off state. This plasmid was constructed to insulate BioBrick parts from read-through transcription coming from the backbone. | |
| + | '''Double Forward and Backwards Terminator Assembly'''<br> | ||
| + | The double terminators were assembled from smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored. | ||
===Source=== | ===Source=== | ||
Revision as of 17:46, 19 October 2006
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No part name specified with partinfo tag.
Design Notes
Purpose
Read-through transcription is extremely problematic when the usefulness of a device depends upon an off state. This plasmid was constructed to insulate BioBrick parts from read-through transcription coming from the backbone.
Double Forward and Backwards Terminator Assembly
The double terminators were assembled from smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
Source
Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos