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<partinfo>BBa_K1497004 short</partinfo>
<partinfo>BBa_K1497004 short</partinfo>
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− The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
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− <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
− Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.</span></p>
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− For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
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Revision as of 11:30, 11 October 2014
B0034-CHS - Chalcone Synthase from Gerbera with strong RBS
Sequence and Features
Assembly Compatibility:
10
12INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 543
21INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 552
23
25INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 523
Illegal NgoMIV site found at 1099
1000INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 472
