Difference between revisions of "Part:BBa K1497001"
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===Usage and Biology===
 
===Usage and Biology===
  
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The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.  
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The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1497001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1497001 SequenceAndFeatures</partinfo>

Revision as of 10:59, 11 October 2014

CHS - Chalcone Synthase from Gerbera

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.


Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.

Usage and Biology

The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon (K1497007).
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454