Difference between revisions of "Part:BBa K1497001"
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The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
 
The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
  
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The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.
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===Usage and Biology===
 
===Usage and Biology===
  
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The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1497001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1497001 SequenceAndFeatures</partinfo>

Revision as of 10:53, 11 October 2014

CHS - Chalcone Synthase from Gerbera

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.


Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.

Usage and Biology

The iGEM Team TU Darmstadt 2014 used the CHS and verified the function of the CHS in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454