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The chalcone isomerse catalyzes the reaction from naringenin chalcone to naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.
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The iGEM Team TU Darmstadt 2014 used the CHI and verified the function of the CHI in their naringenin operon <a href="/Part:BBa_K1497007">(K1497007)</a>.
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      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
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Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. The CHI has a high E-value and produce only S-naringenin</span></p>
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The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.
 
  
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For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
 
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:17, 11 October 2014

B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

The chalcone isomerse catalyzes the reaction from naringenin chalcone to naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.

The iGEM Team TU Darmstadt 2014 used the CHI and verified the function of the CHI in their naringenin operon (K1497007).


Figure 1 Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. The CHI has a high E-value and produce only S-naringenin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706