Difference between revisions of "Part:BBa K1445002"

Revision as of 03:02, 11 October 2014

High copy BioBricked M13 phagemid vector

This biobrick backbone has the high copy number pUC19 origin of replication and an ampicillin resistance marker.

Part also contains the M13 origin of replication which allows the phagemid to be packaged into M13 phage. Vector does not contain any expressible phage genes; therefore, a helper phagemid is required for phage coat protein expression.

Vector contains the following features:

           iGEM VF2 and VR sequencing sites
           T7 promoters that flank the MCS
           Translational stop sequences (BBa_B0042) that flank the MCS
           Transcriptional terminator BBa_B0055 upstream of the MCS
           Transcriptional terminator BBa_B0054 downstream of the MCS

Usage and Biology

BBa_K1445002

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2990
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2996
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2990
Illegal BglII site found at 2852
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2990
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2990
Plasmid lacks a suffix.
Illegal XbaI site found at 3005
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1240

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              Transcriptional terminator BBa_B0054 downstream of the MCS
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 ===Usage and Biology===