Difference between revisions of "Part:BBa K1412614:Experience"
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===='''Genetic link stage'''==== | ===='''Genetic link stage'''==== | ||
| + | 1.As a skeleton, TT terminator link with target gene <i>CheZ</i>. | ||
| + | |||
| + | 2.Then link the skeleton to target gene <i>CheZ</i>-TT. | ||
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| + | 3.Next, link RBS-<i>CheZ</i>-TT with promoter Plac. | ||
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| + | ========Genetic link stage==== | ||
[[File:Verification.png |900px]] | [[File:Verification.png |900px]] | ||
Revision as of 02:49, 11 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Protocol
Genetic link stage
1.As a skeleton, TT terminator link with target gene CheZ.
2.Then link the skeleton to target gene CheZ-TT.
3.Next, link RBS-CheZ-TT with promoter Plac.
====Genetic link stage
Characterization stage
1.Transfer the part Plac-RBS-CheZ-TT into E.coli (CheZ knocked out), and coat plates, culture for hours to measure the migration diameter of E.coli.
Applications of BBa_K1412614
User Reviews
UNIQ4974e39d9d9b5e22-partinfo-00000000-QINU UNIQ4974e39d9d9b5e22-partinfo-00000001-QINU