Difference between revisions of "Part:BBa K1481005"
 
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<partinfo>BBa_K1481005 short</partinfo>
 
<partinfo>BBa_K1481005 short</partinfo>
  
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This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP  expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage.
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This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP  expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage using [https://parts.igem.org/Part:BBa_K1481006 BBa_K1481006].
  
 
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Latest revision as of 20:11, 10 October 2014

Strobable rhamnose AND temperature operated transcriptor SR-latch

This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage using BBa_K1481006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 125
    Illegal BamHI site found at 629
    Illegal XhoI site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1924
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1944
    Illegal BsaI.rc site found at 1463
    Illegal BsaI.rc site found at 1827
    Illegal BsaI.rc site found at 2101
    Illegal SapI.rc site found at 2193