Difference between revisions of "Part:BBa K1499402:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1499402 short</partinfo> | <partinfo>BBa_K1499402 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | The sequence for this gene was predicted through peptide mass fingerprinting of ''Polistes dominula'' (European paper wasp) nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for ''E. coli'' expression. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | ''Polistes dominula'' genome. | |
===References=== | ===References=== | ||
+ | ''Polistes dominula'' genome: | ||
+ | http://goblinx.soic.indiana.edu/PdomGDB | ||
+ | |||
+ | Studies of amino acid composition of nest protein in other ''Polistes'' species: | ||
+ | http://link.springer.com/article/10.1007/PL00001733 | ||
+ | http://link.springer.com/article/10.1007/s101640070003 | ||
+ | http://link.springer.com/article/10.1007/BF00997166 | ||
+ | http://link.springer.com/article/10.1007/BF00982111 |
Revision as of 17:55, 10 October 2014
Uncharacterized wasp protein 1 (PdomMRNAr1.2-02758.1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 278
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this gene was predicted through peptide mass fingerprinting of Polistes dominula (European paper wasp) nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for E. coli expression.
Source
Polistes dominula genome.
References
Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB
Studies of amino acid composition of nest protein in other Polistes species: http://link.springer.com/article/10.1007/PL00001733 http://link.springer.com/article/10.1007/s101640070003 http://link.springer.com/article/10.1007/BF00997166 http://link.springer.com/article/10.1007/BF00982111