Difference between revisions of "Part:BBa K1499402:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1499402 short</partinfo>
 
<partinfo>BBa_K1499402 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The sequence for this gene was predicted through peptide mass fingerprinting of ''Polistes dominula'' (European paper wasp) nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for ''E. coli'' expression.
 
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===Source===
 
===Source===
  
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''Polistes dominula'' genome.
  
 
===References===
 
===References===
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''Polistes dominula'' genome:
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http://goblinx.soic.indiana.edu/PdomGDB
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Studies of amino acid composition of nest protein in other ''Polistes'' species:
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http://link.springer.com/article/10.1007/PL00001733
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http://link.springer.com/article/10.1007/s101640070003
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http://link.springer.com/article/10.1007/BF00997166
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http://link.springer.com/article/10.1007/BF00982111

Revision as of 17:55, 10 October 2014

Uncharacterized wasp protein 1 (PdomMRNAr1.2-02758.1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 278
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this gene was predicted through peptide mass fingerprinting of Polistes dominula (European paper wasp) nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for E. coli expression.

Source

Polistes dominula genome.

References

Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB

Studies of amino acid composition of nest protein in other Polistes species: http://link.springer.com/article/10.1007/PL00001733 http://link.springer.com/article/10.1007/s101640070003 http://link.springer.com/article/10.1007/BF00997166 http://link.springer.com/article/10.1007/BF00982111