Difference between revisions of "Part:BBa K1477014:Experience"

(Applications of BBa_K1477014)
(Applications of BBa_K1477014)
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===Applications of BBa_K1477014===
 
===Applications of BBa_K1477014===
 
"http://i.imgur.com/FwXzsYX.jpg
 
"http://i.imgur.com/FwXzsYX.jpg
According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2600 base pairs with a pSB1C3 bckbone. This matches our quantitative data.
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<p>
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According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2600 base pairs with a pSB1C3 bckbone. This matches our quantitative data.</P>
  
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http://i.imgur.com/kwgTE7u.png
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<p>
 
We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.
 
We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.
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</P>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:07, 10 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1477014

"http://i.imgur.com/FwXzsYX.jpg

According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2600 base pairs with a pSB1C3 bckbone. This matches our quantitative data.

http://i.imgur.com/kwgTE7u.png

We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.

User Reviews

UNIQbafb042a4c3e76a3-partinfo-00000000-QINU UNIQbafb042a4c3e76a3-partinfo-00000001-QINU