Difference between revisions of "Part:BBa K1484214:Design"

 
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===Design Notes===
 
===Design Notes===
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5UTR
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Transcription start site unknown. This contains the 5'UTR of the EF1a gene.
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+
  
 +
Restriction sites added to enable PlantSyntax compatible assembly
  
 
===Source===
 
===Source===
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+
This promoter was discovered by sequence comparison with EF1 type promoters in P. patens and A. thaliana. This version was isolated by PCR from genomic DNA, and modified by to remove illegal restriction sites.
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MP Genome
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===References===
 
===References===

Latest revision as of 15:39, 10 October 2014


MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 146
    Illegal BglII site found at 234
    Illegal XhoI site found at 1479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1751
    Illegal SapI.rc site found at 1350


Design Notes

Transcription start site unknown. This contains the 5'UTR of the EF1a gene.

Restriction sites added to enable PlantSyntax compatible assembly

Source

This promoter was discovered by sequence comparison with EF1 type promoters in P. patens and A. thaliana. This version was isolated by PCR from genomic DNA, and modified by to remove illegal restriction sites.

References