Difference between revisions of "Part:BBa K1321304"
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<partinfo>BBa_K1321304 short</partinfo>
 
<partinfo>BBa_K1321304 short</partinfo>
  
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This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used it as a shuttle vector for G.xylinus genetic engineering
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This part contains Anderson promoter J23117 and RFP coding device in pSEVA321-BB backbone. pSEVA321-BB is a broad host range plasmid, capable of replication in E.coli and several other G- bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used this part to express RFP in G.xylinus.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:40, 10 October 2014

pSEVA321-BB with J23117-RFP

This part contains Anderson promoter J23117 and RFP coding device in pSEVA321-BB backbone. pSEVA321-BB is a broad host range plasmid, capable of replication in E.coli and several other G- bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used this part to express RFP in G.xylinus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4476
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4476
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NotI site found at 913
    Illegal NotI site found at 4482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4476
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4476
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4476
    Illegal XbaI site found at 4491
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NgoMIV site found at 2095
    Illegal NgoMIV site found at 2980
    Illegal NgoMIV site found at 4091
    Illegal NgoMIV site found at 4215
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]