Difference between revisions of "Part:BBa K1455006"
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This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time.
 
This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time.
  
−
AmcilCP and PSP3 are constitutively expressing by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again.
+
AmcilCP and PSP3 are expressing constitutively  by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again.
  
−
PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family ( (parts BBa_I746360 to BBa_I746365).
+
In addition, PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family (parts BBa_I746360 to BBa_I746365).
  
−
TetR is, theoretically, able to turn off all the system and be turned on again with tetracycline (or analogues), so this device can be useful as a part of a complex regulation circuit.
+
Is important to highlight that TetR is, theoretically, able to turn off all the system, and after the system can be turned on again by the addition of tetracycline (or analogues), so this device could be useful as a part of a more complex regulation circuit.
  
 
Here is an example of an ''E: coli'' DH5α culture carrying this device:
 
Here is an example of an ''E: coli'' DH5α culture carrying this device:

Revision as of 01:16, 10 October 2014

psid - pTet - AmilCP - pag activator (PSP3) Positive feedback

This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time.

AmcilCP and PSP3 are expressing constitutively by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again.

In addition, PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family (parts BBa_I746360 to BBa_I746365).

Is important to highlight that TetR is, theoretically, able to turn off all the system, and after the system can be turned on again by the addition of tetracycline (or analogues), so this device could be useful as a part of a more complex regulation circuit.

Here is an example of an E: coli DH5α culture carrying this device:

300px

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]