Difference between revisions of "Part:BBa K1321302"

Revision as of 21:16, 9 October 2014

pSEVA331-BB with J23100-RFP

This is pSEVA331 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA331 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3776
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NotI site found at 913
Illegal NotI site found at 3782
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3776
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 3776
Illegal XbaI site found at 3791
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NgoMIV site found at 2095
Illegal AgeI site found at 616
Illegal AgeI site found at 728
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1321302 short</partinfo>
-x
+This is pSEVA331 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA331 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.
 <!-- Add more about the biology of this part here